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The complete check-up test requires 10 to 12 hours of fasting. It is preferable to perform your test in the early morning hours without any special physical activity.

No, it's not mandatory. However, to facilitate scheduling and the sample collection process, as well as to optimize your valuable time, we recommend booking an appointment through the laboratory's online scheduling system before your visit.

This sampling method has gained attention due to its ease of transportation and lack of special requirements.

For infants, the proper sampling site is the lateral part of the foot sole, various capillaries, or standard venipuncture sites (Figure 1). We prepare the infant's sampling site by massaging or applying a warm towel, disinfect the area with alcohol and wait briefly for it to dry, then puncture the site with a lancet. The first drop of blood is wiped away with sterile gauze, and we allow a large blood drop to form. Gently touch the first circle on the paper (Figure 2) with the blood drop until the blood is absorbed and fills the entire circle. Repeat this process for several more circles until sampling is complete. If blood is drawn from a capillary or vein using a syringe, carefully place one drop of blood in the center of each circle on the paper until the circle is completely filled (Figure 3).

The blood collection papers should dry for about 2-3 hours in a dry place completely away from light.

• The sampled paper should be placed in a paper envelope and sent.
• Sample stability: 7 days at room temperature, 1 month refrigerated, 3 months frozen.

1. Fasting is NOT required for the IGRA test.

2. For sample collection, four special QFT tubes are used:

   • Nil tube (gray cap)

   • TB antigen tube 1 (green cap)

   • Mitogen tube 2 (purple cap)

   • TB antigen tube (yellow cap)

3. Collection methods:

   • For vacuum systems: Fill to black line (exactly 1 mL)

   • For syringe systems: Add blood to black line (exactly 1 mL)

   • Mix immediately by inverting 10 times

4. If QFT tubes unavailable:

   • Collect 5 mL lithium heparin blood

   • Store/transport at 22±5°C

   • Must transfer to QFT tubes within 16 hours

5. Incubation:

   • Incubate at 37°C for 16-24 hours

   • Centrifuge at 2000-3000 RCF for 15 minutes

   • Repeat at higher speed if plasma not separated

6. Storage:

   • Plasma stable for:

     • 28 days at 2-8°C

     • 3 months at -20°C

   • Label separated plasma tubes with original cap color

1. Mix the collected fluid sample by inverting the container. For volumes <5 mL, add an equal volume of 70% ethanol and mix thoroughly. For volumes >5 mL, mix 2 mL sample with 2 mL 70% ethanol in a test tube and centrifuge (15 min at 3000 rpm for dilute fluids like urine/CSF; 10 min for viscous fluids).

2. Prepare ≥3 thin oval smears from the sediment. Air-dry at room temperature (no fixative required). Label each slide with patient name, date, and sample ID.

3. Prepared slides can be submitted together and remain stable at room temperature for ≥1 month.

4. If centrifugation is unavailable:

   • Add 70% ethanol as above

   • Refrigerate (2-8°C)

   • Process slides ASAP or ship refrigerated

5. Maximum storage:

   • CSF: 1 hr RT

   • Other fluids: 3 hrs RT or 24 hrs refrigerated

   • Ethanol-preserved: 48 hrs refrigerated

1. Gently mix the collected fluid sample by inverting or swirling the container. For samples less than 5 mL, add an equal volume of 70% ethanol and mix well. For samples greater than 5 mL, mix 2 mL of sample with 2 mL of 70% ethanol in a test tube and centrifuge (15 minutes at 3000 rpm for dilute fluids like urine or CSF; 10 minutes at same speed for viscous fluids).

2. Prepare at least 3 thin (oval-shaped) smears from the sediment and air-dry at room temperature (no fixative required). Label each slide with patient name, date, and sample number.

3. Prepared slides can be stored at room temperature for up to 1 month and may be submitted together.

4. If centrifugation is unavailable, add 70% ethanol as described, refrigerate (2-8°C), and process slides as soon as possible.

5. Maximum storage:

   • CSF: 1 hour room temp

   • Other fluids: 3 hours room temp or 24 hours refrigerated

   • Alcohol-preserved samples: 48 hours refrigerated